BioPrep
TotalExtract Protein Extraction Kit
SKU: BP-TPEX-100
Universal protein extraction kit for cells, tissue, and biofluid samples — optimized for downstream LC-MS and 2D gel workflows.
Applications
Proteomic sample prepWestern blotELISA2D gel input
Overview
TotalExtract delivers reproducible, high-yield protein extraction from a wide variety of biological matrices without detergent contamination that could compromise MS ionization. The proprietary lysis buffer formulation maintains native-like protein integrity while enabling complete extraction of cytoplasmic, membrane, and nuclear fractions.
Specifications
| Yield (Cell Lysate) | ≥ 3 mg/mL from 10^6 HeLa cells |
|---|---|
| Recovery | > 95% for most cytoplasmic proteins |
| MS Compatibility | Yes — detergent-free formulation |
| Sample Input | Cells, fresh/frozen tissue, serum, plasma |
| Reactions | 100 extractions |